Successful Outcome in a patient of Recurrent Implantation failures using Non invasive PGT-A : a case report

Abstract

Aim:To perform and test accuracy of non invasive chromosome screening PGT A for a couple with history of recurrent implantation failure with male factor infertility .

Settings: In vitro fertilization (ICSI-TESA) procedure and noninvasive PGT A were performed at Bloom IVF, Mumbai, India.

Methods: The Couple undergoing ICSI had previous history of four failed embryo transfers. Total 10 oocytes were retrieved after standard antagonist stimulation protocol. Oocytes were fertilized using ICSI,  after performing TESA on the male partner. The resulting embryos on day 5 were subjected to non-invasive PGT testing with spent culture medium. We collected the spent culture media samples on D5 and performed whole genome amplification (WGA) and sequencing by multiple annealing and looping-based amplification cycles (MALBAC) and next generation sequencing (NGS). The MALBAC NGS protocol has been previously validated in performing PGT with cleavage-stage and blastocyst-stage biopsies.

Results: Out of two tested blastocysts using spent medium, one was euploid and the other one was aneuploid. The transfer of the single euploid embryo, resulted in pregnancy and live birth.

Conclusion: We hereby report the birth of a healthy baby after non invasive PGT A testing for the first time in India